Isolation of microorganisms and lipase production study using agricultural residues
DOI:
https://doi.org/10.14808/sci.plena.2016.054214Keywords:
Lipase, Microorganism, IsolationAbstract
Lipases are enzymes that have been highlighted in the biotechnology scenario, mainly due to their ability to catalyze hydrolysis and synthesis reactions. Lipases are produced by microorganisms, animals and plants, but the microbial origin is currently the most widely used industrially. Among the lipase producers microorganisms are filamentous fungi, bacteria and yeasts. This study aimed to isolation and selection of bacteria producing lipase and the enzyme production study using agro industrial wastes. Samples for isolation were collected in a biodiesel pilot plant. Microorganisms were isolated and then subjected to testing for lipase activity. After the selection process, the isolates showed greater enzymatic index (EI) were used for the study of lipase production using bagasse from sugarcane and cassava bagasse. In this studied were isolated 16 microorganisms, where 75% presented lipolytic activity. Among the isolates selected as lipase producers, the maximum production was achieved with the 8A strain when used bagasse cassava for 48 h fermentation.
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